In Plasmodium falciparum the HA-tagged GAPMs (Glideosome Associated Protein with Multiple membrane spans) co-localise with the inner membrane complex protein GAP45 but not the plasma membrane protein MSP119. Immunofluorescence images of PfGAPM1-HA (A & B) and PfGAPM3-HA (C & D) expressing P. falciparum schizonts probed with an anti-HA antibody and antibodies to either the IMC-resident protein PfGAP45 (A & C) or the MSP119 subunit of the plasma membrane protein MSP1 PFI1475w (B & D). PfGAPM1-HA and PfGAPM3-HA co-localize with PfGAP45 PFL1090w throughout development but not with MSP119 particularly during early schizogony.Bullen HE, Tonkin CJ, O'Donnell RA, Tham WH, Papenfuss AT, Gould S, Cowman AF, Crabb BS, Gilson PR. A novel family of apicomplexan glideosome associated proteins with an inner-membrane anchoring role. J Biol Chem. 2009 284:25353-63.

PfM6T proteins localize to the merozoite IMC. Confocal immunofluorescence images demonstrating location of each paralog, as indicated above each image. Images show a separate schizont-infected erythrocyte containing many merozoites; overlay of the red and green channels (rightmost column) shows colocalization of PfM6Ta with each other paralog. Red and green images reflect detection using specific antibodies raised in rabbits and mice, respectively. White scale bars represent 1mm.Rayavara K, Rajapandi T, Wollenberg K, Kabat J, Fischer ER, Desai SA. A complex of three related membrane proteins is conserved on malarial merozoites. Mol Biochem Parasitol. 2009 167:135-43. Copyright Elsevier 2010