Immuno-fluorescence staining of PfSRP54, PfSRP19, PfSRP14 and PfSRP9 in P. falciparum detected by specific anti-PfSRP54, anti-PfSRP19 and anti-PfSRP14 and PfSRP9 sera. SRP polypeptides are expressed in all three blood stages; ring, trophozoite and schizont. The staining was seen in the parasite cytoplasm and to some extent in the nucleus as well.Panchal M, Rawat K, Kumar G, Kibria KM, Singh S, Kalamuddin M, Mohmmed A, Malhotra P, Tuteja R. Plasmodium falciparum signal recognition particle components and anti-parasitic effect of ivermectin in blocking nucleo-cytoplasmic shuttling of SRP. Cell Death Dis. 2014 Jan 16;5:e994.

Co-staining of PfSRP polypeptides with nucleolar marker PfNOP1, ER marker PfBiP and cytoplasmic marker Pfactin. (a, b and c) P. falciparum infected erythrocytes stained with anti-PfSRP antibodies (green), anti-PfNOP1 (red), anti-PfBiP (red) and anti-Pfactin (red) respectively. (d) localization of GFP fused SRP19 (green) with ER tracker (red). DAPI is used for nuclear staining (blue). A considerable overlap in staining was observed between three PfSRP polypeptides; PfSRP19, -14, and -9 with PfNOP1 and with PfBiP protein (a and b, i-iv). Importantly, considerable co-localization of PfSRP54 with PfNOP1 in early stages of parasite development i.e. merozoite and ring stages.Panchal M, Rawat K, Kumar G, Kibria KM, Singh S, Kalamuddin M, Mohmmed A, Malhotra P, Tuteja R. Plasmodium falciparum signal recognition particle components and anti-parasitic effect of ivermectin in blocking nucleo-cytoplasmic shuttling of SRP. Cell Death Dis. 2014 Jan 16;5:e994.

Co-localisation of PfSRP polypeptides. (a), (b) and (c) co-localisation of PfSRP54 (green) with PfSRP 19,-9 and -14 (red), respectively. (d) and (e) co-localisation of PfSRP9 (green) with PfSRP14 (red) and PfSRP19 (red), respectively. (f) co-localisation of PfSRP19 (green) and PfSRP14(red). DAPI is used as a nuclear stain (blue). In comparison to PfSRP54 distribution in the nucleus, staining for PfSRP19, PfSRP14 and PfSRP9 in subnuclear areas was quite intense at the asexual blood stages particularly at the trophozoite stage.Panchal M, Rawat K, Kumar G, Kibria KM, Singh S, Kalamuddin M, Mohmmed A, Malhotra P, Tuteja R. Plasmodium falciparum signal recognition particle components and anti-parasitic effect of ivermectin in blocking nucleo-cytoplasmic shuttling of SRP. Cell Death Dis. 2014 Jan 16;5:e994.