Localization of PfMA by Immuno-electron microscopy. Ultra thin sections of P. falciparum parasites at schizont/merozoite stages were labelled with mouse anti-PfMA or anti-EBA-175 antibodies followed with anti-mouse secondary antibody conjugated with 15nm colloidal gold particle. (A) Labelling with the PfMA antibody was confined only to the micronemes (arrows) in the apical end of the free merozoite and was absent from the rhoptry (Rh) or nucleus (Nu). (B, C) Similarly PfMA staining in the schizonts was observed only in the micronemes and not in the rhoptries. (D, E) A similar pattern of staining in the schizonts was observed with the micronemal marker EBA-175.Hans N, Singh S, Pandey AK, Reddy KS, Gaur D, Chauhan VS. Identification and Characterization of a Novel Plasmodium falciparum Adhesin Involved in Erythrocyte Invasion. PLoS One. 2013 Sep 13;8(9):e74790.

PfMA localization in schizont stages analyzed by fluorescence microscopy. Sub-cellular localization of PfMA was studied by co-immunostaining with surface protein (A), microneme (B), rhoptry (C, D) resident proteins. P. falciparum schizonts were co-immunostained with mouse anti-PfMA (green) and rabbit antibodies against one of the 4 marker proteins (EBA175/PfRH2, ASP, MSP-119) (red). The nuclei of schizont were stained with DAPI (blue) and slides were visualized by fluorescence microscope. All apical marker proteins and PfMA showed punctate staining in schizonts. PfMA was localized in the micronemes as it signal costained with micronemal marker EBA-175. The scale bar indicates 2 μm.Hans N, Singh S, Pandey AK, Reddy KS, Gaur D, Chauhan VS. Identification and Characterization of a Novel Plasmodium falciparum Adhesin Involved in Erythrocyte Invasion. PLoS One. 2013 8(9):e74790.