PKNH_0716900 gamma-glutamylcysteine synthetase, putative
Disruptability [+]
| Species | Disruptability | Reference | Submitter | |
|---|---|---|---|---|
| P. berghei ANKA |
Possible |
RMgm-1417 | Imported from RMgmDB | |
| P. berghei ANKA |
Possible |
RMgm-204 | Imported from RMgmDB | |
| P. falciparum 3D7 |
Refractory |
26060916 | Theo Sanderson, Wellcome Trust Sanger Institute | |
| P. falciparum 3D7 |
Refractory |
USF piggyBac screen (Insert. mut.) | USF PiggyBac Screen | |
Mutant phenotypes [+]
| Species | Stage | Phenotype | Reference | Submitter |
|---|---|---|---|---|
| P. berghei ANKA | Asexual |
Difference from wild-type |
RMgm-1417
Reduced growth of asexual blood stages in mice |
Imported from RMgmDB |
| P. berghei ANKA | Asexual |
Difference from wild-type |
RMgm-204
Blood stages show a small decrease in the growth rate in mice. |
Imported from RMgmDB |
| P. berghei ANKA | Gametocyte |
No difference |
RMgm-204 | Imported from RMgmDB |
| P. berghei ANKA | Ookinete |
Difference from wild-type |
RMgm-204
Normal gametocyte production. The ookinete production in Anopheles stephensi mosquitoes was reduced by 30-40% as compared to wild type parasites. Ookinetes had a reduced capacity to develop into (mature) oocysts. |
Imported from RMgmDB |
| P. berghei ANKA | Oocyst |
Difference from wild-type |
RMgm-204
Ookinetes had a reduced capacity to develop into (mature) oocysts. At two days after feeding of mosquitoes the number of young oocysts (stained with anti-P28 antibodies) was reduced by 70-90% when compared to wild type. At day 12 after feeding the oocysts numbers were reduced by 65-85%. Day 12 oocysts were significantly smaller than wild type oocysts and no formation of sporozoites was observed. |
Imported from RMgmDB |
| P. berghei ANKA | Sporozoite |
Difference from wild-type |
RMgm-204
No formation of sporozoites |
Imported from RMgmDB |
Imaging data (from Malaria Metabolic Pathways)
Localization of Plasmodium falciparum c-glutamylcysteine synthetase–green fluorescent protein (PfcGCSGFP) and glutathione synthetase-GFP (PfGS-GFP). P. falciparum GS and gGCS C-terminally tagged with GFP were expressed in P. falciparum D10 erythrocytic stages. Fluorescence was analyzed using an Axioskop-2 mot plus microscope (Zeiss) equipped with a Hamamatsu C4742-95 CCD camera and was shown to be present throughout the cytoplasm of both transfected parasite lines, suggesting that GSH biosynthesis is cytoplasmic. No fluorescence is seen in the digestive vacuoles (DV) of the parasites or the erythrocyte host cell (E). E, erythrocyte; P, parasite; DV, digestive vacuole with hemozoin crystals.Patzewitz EM, Salcedo-Sora JE, Wong EH, Sethia S, Stocks PA, Maughan SC, Murray JA, Krishna S, Bray PG, Ward SA, Müller S. Glutathione Transport: A New Role for PfCRT in Chloroquine Resistance. Antioxid Redox Signal. 2012 19(7):683-95 PMID:
See original on MMPMore information
| PlasmoDB | PKNH_0716900 |
| GeneDB | PKNH_0716900 |
| Malaria Metabolic Pathways | Localisation images Pathways mapped to |
| Previous ID(s) | PK9_1750w, PKH_071610 |
| Orthologs | PBANKA_0819800 , PCHAS_0820100 , PF3D7_0918900 , PVP01_0717300 , PVX_099360 , PY17X_0823100 |
| Google Scholar | Search for all mentions of this gene |