PfnPrx is a nuclear protein. Top) Immuno-fluorescence using the mouse monoclonal anti-PfnPrx antibody demonstrates localisation of PfnPrx in the nucleus throughout the erythrocytic stage. Bottom) PfnPrx does not localise to the tight junction of merozoites in the process of invasion. Immunofluorescence with mouse monoclonal anti-nPrx and rabbit anti-RON4 antibody shows that PfnPrx is restricted to the nucleus in parasites invading a red blood cell. Richard D, Bartfai R, Volz J, Ralph SA, Muller S, Stunnenberg HG, Cowman AF. A genome-wide chromatin-associated nuclear from the malaria parasite Plasmodium falciparum. J Biol Chem. 2011 286(13):11746-55

A. Endogenously tagging nPrx confirms its status as a nuclear protein. Epifluorescence microscopy on the 3D7nPrx-3’GFP line demonstrates that the tagged nPrx localises to the nucleus in all blood stages.B. PfnPrx is a nuclear protein. Immunoelectron microscopy using the rabbit anti-PfnPrx antibody on schizont stage parasites reveals that PfnPrx is in the electron dense nuclear periphery. The image shows a single merozoite located within a schizont.Richard D, Bartfai R, Volz J, Ralph SA, Muller S, Stunnenberg HG, Cowman AF. A genome-wide chromatin-associated nuclear from the malaria parasite Plasmodium falciparum. J Biol Chem. 2011 Jan 31. [Epub ahead of print]