Sub-cellular localization of enzymes involved in fumarate to aspartate conversion pathway. Indirect immunofluorescence analysis of parasitized erythrocytes with anti-PfAAT and anti-PfHsp60 antisera. Anti-mouse and anti-rabbit IgG antibodies conjugated with Alexa-Fluor dyes were used as secondary antibodies. Hoechst was used as the nuclear stain. PfAAT is present in the cytosol. No co localization of PfAAT (red) was found with the mitochondrial marker, PfHSP60.Bulusu V, Jayaraman V, Balaram H. Metabolic fate of fumarate, a side product of purine salvage pathway in the intraerythrocytic stages of Plasmodium falciparum. J Biol Chem. 2011 286(11):9236-45.

Localization of PfAspAT to the cytosol of the parasite. Localization of AspAT within P. falciparum. (a) A chimera protein consisting of the PfAspAT N-terminal region (67 aa residues) fused to GFP and (c) a fusion protein consisting of the PfAspAT C-terminal region attached to the C-terminal end of GFP were overexpressed in P. falciparum using the pARL1a expression vector. The developmental blood stages of the parasites were analyzed by fluorescent light microscopy using an Axioskop 2 plus microscope (Zeiss, Germany). (I) Ring stage; (II) trophozoite stage; (III) schizont stage. Phase, P. falciparum live image; Hoechst, staining of the parasite's nucleus; GFP, image taken using the GFP channel; Merge, merging of all images. GFP-tagged PfAspAT localizes to the cytosol in vivo.Wrenger C, Müller IB, Schifferdecker AJ, Jain R, Jordanova R, Groves MR. Specific Inhibition of the Aspartate Aminotransferase of Plasmodium falciparum. J Mol Biol. 2010 405:956-71. Copyright Elsevier 2011