"As shown in Figure 4A and Supplementary Figure 4, in the first 2 IDCs without aTc, PfmtRPS12 parasites did not exhibit a loss in parasitemia or any detectable abnormality in morphology; however, the parasites started to show a decrease in growth rate in the 3rd IDC (~ 20% loss) and more noticeably in the 4th IDC (~ 50% loss). After withdrawal of aTc for 4 or 5 IDCs, some PfmtRPS12 parasites displayed noticeable abnormalities in morphology (Supplementary Figure 4A). Despite these defects in growth rate and parasite morphology, PfmtRPS12 parasites were able to continuously grow after aTc removal for longer than 5 IDCs (data not shown)."