Upper panel: Specific labeling with mouse anti-Vsp4 of the cytosol. ER, endoplasmic reticulum; N, nucleus; Pf, Plasmodium falciparum; DV, digestive vacuole. Bar, 0.15 mm. Lower panel: Mutant GFP-PfVps4 localizes to punctuate structures when transiently expressed in P. falciparum. (B) Pattern of GFP fluorescence in stable line of P. falciparum expressing GFP-PfVps4-WT. Corresponding phase contrast images are illustrated. (C) Pattern of GFP fluorescence in P. falciparum transiently transfected with GFP-PfVps4-E214Q, mutated to block ATP hydrolysis. Arrows indicate the punctuate structures.Yang M, Coppens I, Wormsley S, Baevova P, Hoppe HC, Joiner KA. The Plasmodium falciparum Vps4 homolog mediates multivesicular body formation. J Cell Sci. 2004 117:3831-8.