Localization of the N-terminal extension of Plasmodium falciparum cytidine diphosphate diacylglycerole synthase (PfCDS). (A) Co-localization studies by immunofluorescence assay (a) The native N-terminal extension of PfCDS is labeled in red with antiserum N26 and SERA protein, a parasitophorous vacuole marker, is labeled in green. (b) Live GFP fluorescence of the N-terminal extension of PfCDS fused to GFP (PfCDS-N-GFP). Nuclei were stained in blue with DAPI. Differential interference contrast (DIC) and merged images are shown. Shastri S, Zeeman AM, Berry L, Verburgh RJ, Braun-Breton C, Thomas AW, Gannoun-Zaki L, Kocken CH, Vial HJ. Plasmodium CDP-DAG synthase: An atypical gene with an essential N-terminal extension. Int J Parasitol. 2010 40:1257-1268. Copyright Elsevier 2010.