PF3D7_1359300 exosome complex exonuclease RRP44 (DIS3)
Disruptability [+]
| Species | Disruptability | Reference | Submitter | |
|---|---|---|---|---|
| P. falciparum 3D7 |
Refractory |
30235972 | Theo Sanderson, Google AI | |
| P. falciparum 3D7 |
Possible |
USF piggyBac screen (Insert. mut.) | USF PiggyBac Screen | |
| P. berghei ANKA |
Refractory |
PlasmoGEM (Barseq) | PlasmoGEM | |
Mutant phenotypes [+]
| Species | Stage | Phenotype | Reference | Submitter |
|---|---|---|---|---|
| P. falciparum 3D7 | Asexual |
Transcriptional effect |
30235972 Di-cre approach with loxP-intron. Incomplete deletion, an increase in especially antisense transcripts. Likely a substantial growth reduction given failure to obtain clones by limiting dilution. |
Theo Sanderson, Google AI |
| P. falciparum 3D7 | Asexual |
Difference from wild-type |
32079470 (Knock down)
'by gene knock-down strategy, we showed that the cooperation of RNA exosome catalytic subunit PfDis3 and PfMtr4 cofactor played a major role in ns-ncRNAs degradation' |
Theo Sanderson, Francis Crick Institute |
Imaging data (from Malaria Metabolic Pathways)
Coimmunofluorescence assay of three plasmodial exoribonucleases and a core exosome member, PfRrp45, in ring-stage wild-type 3D7 parasites. The rabbit anti-PfRrp45, anti-PfRNase II, anti-PfRrp6 and mouse anti-PfDis3 antibodies were used in these assays. DAPI, 49,6-diamidino-2-phenylindole. Scale bar, 1 mm. Immunofluorescence assay (IFA) analysis revealed that PfRrp6 and PfRNase II localize as distinct foci at the nuclear periphery and that PfDis3 is localized in the cytoplasm adjacent to the nucleus at the ring stage .Zhang Q, Siegel TN, Martins RM, Wang F, Cao J, Gao Q, Cheng X, Jiang L, Hon CC, Scheidig-Benatar C, Sakamoto H, Turner L, Jensen AT, Claes A, Guizetti J, Malmquist NA, Scherf A. Exonuclease-mediated degradation of nascent RNA silences genes linked to severe malaria. Nature. 2014 Jun 29. PMID:
See original on MMPMore information
| PlasmoDB | PF3D7_1359300 |
| GeneDB | PF3D7_1359300 |
| Malaria Metabolic Pathways | Localisation images Pathways mapped to |
| Previous ID(s) | MAL13P1.289 |
| Orthologs | PBANKA_1135600 , PCHAS_1135100 , PKNH_1112000 , PVP01_1112600 , PVX_114935 , PY17X_1137100 |
| Google Scholar | Search for all mentions of this gene |