The EBA140 and EBA175 proteins colocalize in in the micronemes,of merozoites. Smears of schizonts or free merozoites of the D10 and E12 parasites are shown. Parasites were reacted with a mixture of anti-EBA140 and anti-EBA175 antibodies, followed by a mixture of FITC labelled anti-mouse and rhodamine-labelled anti-rabbit antibodies. ‘Phase’ shows the brightfield image, whereas ‘merge’ shows the red and green images overlaid.Thompson JK, Triglia T, Reed MB, Cowman AF. A novel ligand from Plasmodium falciparum that binds to a sialic acid-containing receptor on the surface of human erythrocytes. Mol Microbiol. 2001 41:47-58. Copyright John Wiley & Sons Ltd. 2010.

MAEBL localized with rhoptry proteins and on the merozoite surface and not with microneme proteins. Dual-label indirect immunofluorescence assays analyzed by scanning confocal laser microscopy are shown in each row of images, using primary antibodies against a rhoptry and microneme protein. In each row, the immunolocalization pattern for the first protein listed is in green in the first left-hand panel and the second protein listed is the second panel shown in red. The third panel is the merged image of the first two panels, the final right-hand panel is the brightfield image. Color was assigned electronically. Markers: RhopH2 PFI1445w; EBA-175 MAL7P1.176; RAP1 PFE0080c.Blair PL, Kappe SH, Maciel JE, Balu B, Adams JH. Plasmodium falciparum MAEBL is a unique member of the ebl family. Mol Biochem Parasitol. 2002 122:35-44. Copyright Elsevier 2009.