PF3D7_1235700 ATP synthase subunit beta, mitochondrial
Disruptability [+]
| Species | Disruptability | Reference | Submitter | |
|---|---|---|---|---|
| P. berghei ANKA |
Possible |
RMgm-1241 | Imported from RMgmDB | |
| P. berghei ANKA |
Possible |
RMgm-1240 | Imported from RMgmDB | |
| P. berghei ANKA |
Possible |
RMgm-1239 | Imported from RMgmDB | |
| P. berghei ANKA |
Possible |
PlasmoGEM (Barseq) | PlasmoGEM | |
Mutant phenotypes [+]
| Species | Stage | Phenotype | Reference | Submitter |
|---|---|---|---|---|
| P. berghei ANKA | Asexual |
Difference from wild-type |
RMgm-1241
Slightly reduced growth rate of blood stages |
Imported from RMgmDB |
| P. berghei ANKA | Asexual |
Difference from wild-type |
RMgm-1240
Slightly reduced growth rate of blood stages |
Imported from RMgmDB |
| P. berghei ANKA | Asexual |
Difference from wild-type |
RMgm-1239
Slightly reduced growth rate of blood stages |
Imported from RMgmDB |
| P. berghei ANKA | Asexual |
Attenuated |
PlasmoGEM (Barseq) | PlasmoGEM |
| P. berghei ANKA | Gametocyte |
Difference from wild-type |
RMgm-1241
Slightly reduced (but not significant) production of gametocytes |
Imported from RMgmDB |
| P. berghei ANKA | Gametocyte |
Difference from wild-type |
RMgm-1240
Slightly reduced (but not significant) production of gametocytes |
Imported from RMgmDB |
| P. berghei ANKA | Gametocyte |
Difference from wild-type |
RMgm-1239
Slightly reduced (but not significant) production of gametocytes |
Imported from RMgmDB |
| P. berghei ANKA | Ookinete |
Difference from wild-type |
RMgm-1241
In vitro: Comparable activation of female gametocytes and production of zygotes, and a slight, but significant decrease in ookinete conversion rates for PbATPKO in comparison with wild type parasites. In vivo (mosquito midgut): The proportion of PbATPKO activated females was substantially reduced in vivo (under 2%) compared with wild type (40%). Similarly, the PbATPKO line generated far fewer zygotes (0%) and ookinetes (0%) in vivo. Failure to activate, generate zygotes, and mature to ookinetes by the PbATPKO female gametocytes in vivo was also evident in the preponderance of aberrant females in vivo (90%) compared with in vitro (1%). No ookinetes were observed at 22 h. |
Imported from RMgmDB |
| P. berghei ANKA | Ookinete |
Difference from wild-type |
RMgm-1240
In vitro: Comparable activation of female gametocytes and production of zygotes, and a slight, but significant decrease in ookinete conversion rates for PbATPKO in comparison with wild type parasites. In vivo (mosquito midgut): The proportion of PbATPKO activated females was substantially reduced in vivo (under 2%) compared with wild type (40%). Similarly, the PbATPKO line generated far fewer zygotes (0%) and ookinetes (0%) in vivo. Failure to activate, generate zygotes, and mature to ookinetes by the PbATPKO female gametocytes in vivo was also evident in the preponderance of aberrant females in vivo (90%) compared with in vitro (1%). No ookinetes were observed at 22 h. |
Imported from RMgmDB |
| P. berghei ANKA | Ookinete |
Difference from wild-type |
RMgm-1239
In vitro: Comparable activation of female gametocytes and production of zygotes, and a slight, but significant decrease in ookinete conversion rates for PbATPKO in comparison with wild type parasites. In vivo (mosquito midgut): The proportion of PbATPKO activated females was substantially reduced in vivo (under 2%) compared with wild type (40%). Similarly, the PbATPKO line generated far fewer zygotes (0%) and ookinetes (0%) in vivo. Failure to activate, generate zygotes, and mature to ookinetes by the PbATPKO female gametocytes in vivo was also evident in the preponderance of aberrant females in vivo (90%) compared with in vitro (1%). No ookinetes were observed at 22 h. |
Imported from RMgmDB |
| P. berghei ANKA | Oocyst |
Difference from wild-type |
RMgm-1241
No formation of oocysts |
Imported from RMgmDB |
| P. berghei ANKA | Oocyst |
Difference from wild-type |
RMgm-1240
No formation of oocysts |
Imported from RMgmDB |
| P. berghei ANKA | Oocyst |
Difference from wild-type |
RMgm-1239
No formation of oocysts |
Imported from RMgmDB |
| P. berghei ANKA | Sporozoite |
Difference from wild-type |
RMgm-1241
No formation of oocysts; no formation of sporozoites |
Imported from RMgmDB |
| P. berghei ANKA | Sporozoite |
Difference from wild-type |
RMgm-1240
No formation of oocysts; no formation of sporozoites |
Imported from RMgmDB |
| P. berghei ANKA | Sporozoite |
Difference from wild-type |
RMgm-1239
No formation of oocysts; no formation of sporozoites |
Imported from RMgmDB |
Imaging data (from Malaria Metabolic Pathways)
Localization of epitope-tagged ATP synthase subunits detected using immuno-fluoresence microscopy. Immunofluoresence of alpha, gamma, delta, epsilon, OSCP and c subunits expressed under control of the mRPL2 promoter. Expression of the beta subunit was under the control of its own promoter. Green fluorescence: Anti-HA antibody (AlexaFluor 488-conjugated secondary antibody). Red fluorescence: Mitotracker Red CM-H2XRos. Blue fluorescence: DAPI (4'-6-Diamidino-2-phenylindole). DIC: Differential interference contrast microscopy.Balabaskaran Nina P, Morrisey JM, Ganesan SM, Ke H, Pershing AM, Mather MW, Vaidya AB. ATP synthase complex of Plasmodium falciparum: dimeric assembly in mitochondrial membranes and resistance to genetic disruption. J Biol Chem. 2011 286(48):41312-22. PMID:
See original on MMPMore information
| PlasmoDB | PF3D7_1235700 |
| GeneDB | PF3D7_1235700 |
| Malaria Metabolic Pathways | Localisation images Pathways mapped to |
| Previous ID(s) | 2277.t00345, MAL12P1.343, PFL1725w |
| Orthologs | PBANKA_1450300 , PCHAS_1452600 , PKNH_1455400 , PVP01_1453800 , PVX_100735 , PY17X_1452800 |
| Google Scholar | Search for all mentions of this gene |