The PHISTc protein encoded by PFL0045c/PF3D7_1200900 is an exported protein with a non-canonical PEXEL/HT. A. Fluorescence microscopy. The schematic structure of the GFP-tagged proteins is depicted on the left of representative images of iRBCs The ER-signal is shaded blue, the (non-) canonical PEXEL/HT is shaded black and GFP is shaded green. Putative cleavage sites are indicated by an arrow (blue: SP cleavage site; black: Plasmepsin V cleavage site. B. Fluorescence microscopy. A representative time lapse series is given. C. Immunofluorescence analyses. Antibodies against GFP and the Maurer’s clefts protein REX2 were used. Fluorescence of GFPtagged PFL0045c (12009-KMLCEGFP), a protein with a K.L.E variant, was restricted to numerous small dots in the iRBC cytoplasm (A) that moved rapidly (B). Congruently, 12009-KMLCE-GFP did not colocalize with REX2 (C), indicating that PFL0045c is localized to structures that are similar to J-dotsSchulze J, Kwiatkowski M, Borner J, Schlüter H, Bruchhaus I, Burmester T, Spielmann T, Pick C. The Plasmodium falciparum exportome contains non-canonical PEXEL/HT proteins. Mol Microbiol. 2015 Apr 8. [Epub ahead of print] PMID: