Last updated 2 months ago

Disruptability [+]

Species Disruptability Reference Submitter
P. berghei ANKA
Refractory
RMgm-4089 Imported from RMgmDB
P. berghei ANKA
Refractory
PlasmoGEM (Barseq) PlasmoGEM
P. yoelii yoelii 17X
Refractory
RMgm-4376 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Cell cycle arrest
37884813 (Conditional)
\'An inducible knockout of gene function showed that PfAP2-P is essential for trophozoite development, and critical for var gene regulation, merozoite development and parasite egress. Single-cell RNA sequencing and fluorescence-activated cell sorting revealed de-repression of most var genes in Δpfap2-p parasites. Δpfap2-p parasites also overexpress early gametocyte marker genes, indicating a regulatory role in sexual stage conversion.\'
Theo Sanderson, Francis Crick Institute

Imaging data (from Malaria Metabolic Pathways)

PfAP2-I Is a Nuclear Protein that May Bind a Conserved TGCA DNA Motif Upstream of Some Invasion Genes. Live fluorescence microscopy of synchronized parasites shows that PfAP2-I-GFP (see Figure S1B) localizes to the nucleus of trophozoite and schizont stage parasites but is not detected in ring stages. Hoechst was used as a nuclear marker. Live microscopy and nuclear fractionation assays show that PfAP2-I localizes exclusively to the nucleus of trophozoite and schizont stage parasites.Santos JM, Josling G, Ross P, Joshi P, Orchard L, Campbell T, Schieler A, Cristea IM, Llinás M. Red Blood Cell Invasion by the Malaria Parasite Is Coordinated by the PfAP2-I Transcription Factor. Cell Host Microbe. 2017 21(6).

See original on MMP

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