PF3D7_1037100 pyruvate kinase 2 (PyKII)
Disruptability [+]
| Species | Disruptability | Reference | Submitter | |
|---|---|---|---|---|
| P. falciparum 3D7 |
Refractory |
USF piggyBac screen (Insert. mut.) | USF PiggyBac Screen | |
| P. berghei ANKA |
Refractory |
PlasmoGEM (Barseq) | PlasmoGEM | |
Mutant phenotypes [+]
| Species | Stage | Phenotype | Reference | Submitter |
|---|---|---|---|---|
| P. falciparum 3D7 | Asexual |
Attenuated |
32815516 (Conditional)
Conditional system in which mutant was generated with supplementation so that apicoplast is not required. Gene is required for apicoplast maintenance. Conditional mislocalisation led to decreased transcript levels in the apicoplast. |
Theo Sanderson, Francis Crick Institute |
Imaging data (from Malaria Metabolic Pathways)
Anti-PfPyKII and anti-P. falciparum acyl carrier protein (ACP) antibodies detected by AlexaFlour goat anti-mouse 594 (red) and goat anti-rabbit 488 (green) secondary antibodies, respectively. Immunofluoresecnce of P. falciparum ACP antibody shows the apicoplast. Merged images indicate co-localization of PfPyKII and P. falciparum ACP. Nucleus stained by DAPI (blue).Maeda T, Saito T, Harb OS, Roos DS, Takeo S, Suzuki H, Tsuboi T, Takeuchi T, Asai T. Pyruvate kinase type-II isozyme in Plasmodium falciparum localizes to the apicoplast. This article was published in Parasitol Int. 2009 58:101-5. Copyright Elsevier 2009.
See original on MMPMore information
| PlasmoDB | PF3D7_1037100 |
| GeneDB | PF3D7_1037100 |
| Malaria Metabolic Pathways | Localisation images Pathways mapped to |
| Previous ID(s) | PF10_0363 |
| Orthologs | PBANKA_0520000 , PCHAS_0520200 , PKNH_0621600 , PVP01_0621500 , PVX_110905 , PY17X_0521100 |
| Google Scholar | Search for all mentions of this gene |