(2) Immuno-gold electron micrograph showing gold particles (arrow) distributed evenly along the parasitophorous vacuolar membrane (PVM) which surrounds a P. falciparum schizont (S). The presence of gold particles corresponds to the presence of GLURP. (3) immuno-gold electron micrograph of an erythrocytic merozoite (M). Staining (arrow) is on the surface of the merozoite. This indicates that GLURP is on the surface of the newly released merozoite, either as remnants of the parasitophorous vacuolar membrane or as a component of the merozoite membrane. Bar, 0.5 mm. (4) Gametocyte (G). lmmuno-gold staining (arrow) is located in clusters in the cytoplasm of the erythrocyte adjacent to the gametocyte.Borre MB, Dziegiel M, Høgh B, Petersen E, Rieneck K, Riley E, Meis JF, Aikawa M, Nakamura K, Harada M, et al. Primary structure and localization of a conserved immunogenic Plasmodiumfalciparum glutamate rich protein (GLURP) expressed in both the preerythrocytic and erythrocytic stages of the vertebrate life cycle. Mol Biochem Parasitol. 1991 49:119-31. PMID:

Expression and co-localization of proteins of Pfs38 complex on Plasmodium merozoites. Co-localization studies were performed by immunofluorescence assays. Merozoites were labelled with (a) mouse anti-Pfs38 and rabbit anti-GLURP or with (b) mouse anti-Pfs38 and rabbit anti-Pfs41 antibodies or with (c) mouse anti-Pfs38 and rabbit anti-Pfs12 antibodies or with (d) mouse anti-Pfs38 and rabbit anti-PfsMSP165 antibodies. Partial co-localization was observed between Pfs38 and other proteins of 6-Cys complex with co-localization coefficient of 0.75, 0.61, 0.82, and 0.83 for a–d, respectively. Td represent bright field images. Pfs38 partially co-localized with GLURP, Pfs41, Pfs12, and MSP-1, advocating the co-existence of proteins of 6-Cys protein complex on the merozoite surface (Fig. 3a–d). These results were corroborated by cosedimentation analysis of parasite-derived polypeptides. Western blotting and LC–MS/MS analysis of the glycerol gradient fractions revealed the presence of Pfs38, Pfs41, Pfs12, GLURP, MSP-1, and SERA5 in a single fraction, suggesting that these proteins exist in a complex on the parasite.Paul G, Deshmukh A, Kaur I, Rathore S, Dabral S, Panda A, Singh SK, Mohmmed A, Theisen M, Malhotra P. A novel Pfs38 protein complex on the surface of Plasmodium falciparum blood-stage merozoites. Malar J. 2017 16(1):79