Four a/b-hydrolases are exported into the erythrocyte compartment. Immunofluorescence assay (IFA) of trophozoite/early schizont-stage parasites overexpressing GFP-tagged a/b-hydrolases. Parasites were fixed with paraformaldehyde/glutaraldehyde and stained with anti-GFP (green). The nuclei were stained with 4’,6’-iamidino-2-phenylindole (DAPI) (blue). (A) PfEH1-GFP, (B) PfEH2-GFP, (C) PfXL1-GFP, and (D) PfXL2-GFP. Expression was under control of the constitutive HSP86 promoter. The images are representative of at least three independent experiments. Bars=5 mm. DIC, differential interference contrast. For the two a/b-hydrolase familyA members, fluorescence was observed at the periphery of the infected erythrocyte and within the parasite (A and B). In contrast, for the two family B members, fluorescence was diffuse throughout the cytosol of the infected RBC (C and D).Spillman NJ, Dalmia VK, Goldberg DE. Exported Epoxide Hydrolases Modulate Erythrocyte Vasoactive Lipids during Plasmodium falciparum Infection. MBio. 2016 Oct 18;7(5). pii: e01538-16.