Localisation of PHIST:GFP proteins. The left- and right-hand images show GFP localisation and a phase contrast image, respectively. White filled arrows: peripheral GFP puncta; White unfilled arrow: GFP puncta in host erythocyte cytosol. Scale bar, 2 mm. GFP-tagged PF3D7_0401800, PF3D7_0424600, PF3D7_0532400, PF3D7_1102500 and PF3D7_1476200 were all exported to the host cell and displayed a striking localisation at the edge of the host erythrocyte (1A–E), PF3D7 1252700 was clearly peripheral in the host erythrocyte (F). PF3D7_0201600 (G) shows weak accumulation at the erythrocyte periphery, but the majority of the protein was localised in the RBC cytosol. PF3D7_0936600 was localised in the erythrocyte cytosol, not at the host cell periphery (H).Tarr SJ, Moon RW, Hardege I, Osborne AR. A conserved domain targets exported PHISTb family proteins to the periphery of Plasmodium infected erythrocytes. Mol Biochem Parasitol. 2014 196(1):29-40 PMID: 25106850

Localisation of PHIST:GFP proteins. The left- and right-hand images show GFP localisation and a phase contrast image, respectively. White filled arrows: peripheral GFP puncta; White unfilled arrow: GFP puncta in host erythocyte cytosol. Scale bar, 2 mm. GFP-tagged PF3D7_0401800, PF3D7_0424600, PF3D7_0532400, PF3D7_1102500 and PF3D7_1476200 were all exported to the host cell and displayed a striking localisation at the edge of the host erythrocyte (1A–E), PF3D7 1252700 was clearly peripheral in the host erythrocyte (F). PF3D7_0201600 (G) shows weak accumulation at the erythrocyte periphery, but the majority of the protein was localised in the RBC cytosol. PF3D7_0936600 was localised in the erythrocyte cytosol, not at the host cell periphery (H).Tarr SJ, Moon RW, Hardege I, Osborne AR. A conserved domain targets exported PHISTb family proteins to the periphery of Plasmodium infected erythrocytes. Mol Biochem Parasitol. 2014 196(1):29-40