A. IFA of air-dried monolayers of pRBC collected at each of the three indicated time-points. Slides were probed with mouse monoclonal anti-Pf332-DBL (red) and rabbit polyclonal anti-PfMAHRP1 antibodies (green). The parasite was counterstained with DAPI (blue). Scale bar indicates 5 mm. Pf332 displayed a close association with Maurer’s clefts throughout trophozoite maturation and schizogonyB. Air-dried monolayers of BFA+ and BFA2 pRBC were probed with monoclonal anti-Pf332-DBL (red) and polyclonal anti-PfSeryl-tRNA synthetase antibodies (green), or polyclonal anti-Pf332-E200 (red) and polyclonal anti-PfSBP1 antibodies (green). The parasite was counterstained with DAPI (blue). Scale bar indicates 5 mm. Synchronous early ring-stage pRBC were treated with (+) or without BFA for 20 h and inhibition of protein export was verified by IFA. As controls, we included antibodies towards the BFA sensitive SBP1, and the nonexported Seryl-tRNA synthetase. In the absence of BFA, both the anti-Pf332 and anti-SBP1 antibodies stained Maurer’s clefts, whereas the Seryl-tRNA synthetase antibodies only stained the parasiteNilsson S, Angeletti D, Wahlgren M, Chen Q, Moll K. Plasmodium falciparum Antigen 332 Is a Resident Peripheral Membrane Protein of Maurer's Clefts. PLoS One. 2012;7(11):e46980.