Detection and subcellular localization of PfRF1Mit and PfRF2Api. (C-F) Immunofluorescence localization of the RFs in P. falciparum infected erythrocytes by confocal microscopy. PfRF1Mit co-localizes with Mitotracker Red (C), with no overlap seen with the apicoplast marker PfHU (D). PfRF2Api colocalizes with apicoplast-targeted GFP in the P. falciparum D10 ACPleader-GFP line. (E); no overlap of the PfRF2Api signal is seen with Mitotracker Red (F). Nuclear DNA is stained with DAPI.Vaishya S, Kumar V, Gupta A, Siddiqi MI, Habib S. Polypeptide release factors and stop codon recognition in the apicoplast and mitochondrion of Plasmodium falciparum. Mol Microbiol. 2016 Mar 4. [Epub ahead of print]