PF3D7_0611400 SWIB/MDM2 domain-containing protein (SWIB)

Last updated 3 years ago

Disruptability [+]

Species Disruptability Reference Submitter
P. falciparum 3D7
Possible		 USF piggyBac screen (Insert. mut.) USF PiggyBac Screen
P. berghei ANKA
Possible		 PlasmoGEM (Barseq) PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. falciparum 3D7 Asexual
Attenuated		 32019597 (Knock down)
The parasitemia of PfSWIB∆ showed a significant decline at 96 h during in vitro culture compared with the PfSWIB and 3D7 lines (P < 0.0001). qPCR and RNA-seq analysis confirmed that depletion of PfSWIB not only silences upsA, upsC and partial upsB var genes, as well as removes the silencing of partial upsB var genes at the ring stage in PfSWIB∆ line, but also leads to aberrant expression of upsA and partial upsB/upsC var genes at the mature stage of P. falciparum, during a single 48-h life-cycle.
 Theo Sanderson, Francis Crick Institute
P. berghei ANKA Asexual
Attenuated		 PlasmoGEM (Barseq) PlasmoGEM

Imaging data (from Malaria Metabolic Pathways)

Live cellular localization of GFP-tagged PfSWIB within Plasmodium falciparum. A distinct cytoplasmic GFP signal was observed in all life stages under normal conditions, whereas exposure to 41 °C for 2 h resulted in nuclear localization, 30 min after termination of heat stress. This redistributionwas short lived since a nuclear signal was absent 2–24 h afterwards. R ring stage, T trophozoite stage, S schizont stage. Scale bar in bright fieldrepresents 2.5 μmVieira WA, Coetzer TL. Localization and interactions of Plasmodium falciparum SWIB/MDM2 homologues. Malar J. 2016 Jan 20;15(1):32.

See original on MMP

More information

PlasmoDB PF3D7_0611400
GeneDB PF3D7_0611400
Malaria Metabolic Pathways Localisation images
Pathways mapped to
Previous ID(s) 2270.t00200, MAL6P1.116, PFF0560c
Orthologs PBANKA_0109700 , PCHAS_0110300 , PKNH_1139000 , PVP01_1137800 , PVX_113710 , PY17X_0111300
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