Localization of potential SEMP1 interacting proteins. Immunofluorescence assays of MeOH-fixed RBCs infected with 3D7 expressing EMP1/PF3D7_0702500 (PF07_0008)/PF3D7_0601900 (PFF0090w) with a C-terminal 3xHA tag and 3D7 expressing PIESP2 with a C-terminal GFP-tag, co-labelled with mouse a-SEMP1 and rat a-HA (PF3D7_0702500-3xHA & PF3D7_0601900-3xHA) /a-GFP (PIESP2-GFP). For co-labelling of SEMP1-3xHA with REX1 and Pf332, rat a-HA and rabbit a-REX1 / mouse a-Pf332 antibodies were used. All potential interaction partners co-localized at least partially with SEMP1, but in particular with Pf332 or PIESP2 antibodies only a fraction of SEMP1 positive MCs were labelled with either antibody suggesting that distinct subpopulations of MCs exist. Dietz O, Rusch S, Brand F, Mundwiler-Pachlatko E, Gaida A, Voss T, Beck HP. Characterization of the Small Exported Plasmodium falciparum Membrane Protein SEMP1. PLoS One. 2014 9(7):e103272.