Indirect immunofluorescence images of P. falciparum schizonts stained with rabbit IgG (green) induced by 10 viral-vectored vaccines expressing malaria antigens, and negative control vectors lacking a malaria antigen. Nuclei are counterstained with 4,6-diamidino-2-phenylindole (blue). All sera were tested against 3D7 strain parasites, with the exception of anti-PfRH1 for which FVO strain parasites were used. All images to same scale as Giemsa stained image (top left, on which scale bar indicates 5 μm).Douglas AD, Williams AR, Illingworth JJ, Kamuyu G, Biswas S, Goodman AL, Wyllie DH, Crosnier C, Miura K, Wright GJ, Long CA, Osier FH, Marsh K, Turner AV, Hill AV, Draper SJ. The blood-stage malaria antigen PfRH5 is susceptible to vaccine-inducible cross-strain neutralizing antibody. Nat Commun. 2011 2:601.

A. Labeling of CLAG9 in 3D7 and E5KO. Note there is labeling of E5KO (clag9 knockout) rhoptries with mAb7H8/50 that reveals RAP3, but not with anti-CLAG9 specific sera due to gene disruption. Scale bars = 5 mm. B. Trafficking of PfEMP1 and KAHRP by E5KO; labeling was identical to 3D7. Scale bars = 5 mm. CLAG9 was not detected in the knockout clone E5KO. These results show that the clag9 gene product is not directly implicated in cytoadhesion to CD36.Nacer A, Roux E, Pomel S, Scheidig-Benatar C, Sakamoto H, Lafont F, Scherf A, Mattei D. clag9 is not essential for PfEMP1 surface expression in non-cytoadherent Plasmodium falciparum parasites with a chromosome 9 deletion. PLoS One. 2011;6(12):e29039.