I. PFB0340c (PF3D7_0402000) localizes to the parasitophorous vacuole membrane. Fixed smears probed with rat anti-PF3D7_0402000 (0402000) and anti-PfSERA5 (SERA5) to test for co-localization (Merge). Parasite nuclei were identified with the nuclear stain DAPI, and infected erythrocytes imaged in brightfield (BF).II. PF3D7_0402000 colocalizes with a sub-population of 4.1R. Immunofluorescence microscopy of air-dried smears of 3D7 P. falciparum-infected erythrocytes probed with rabbit anti-4.1R (green), rat anti-PF3D7_0402000 (red) and the nuclear dye (DAPI). PF3D7_0402000 and 4.1R colocalize in early (A) and late (B) trophozoite stage parasites.III. A subpopulation of 4.1R is found at the PVM in Plasmodium falciparum-infected erythrocytes. Immunofluorescence microscopy of air-dried smears of 3D7 P. falciparum-infected erythrocytes probed with rabbit anti-4.1R (green). 4.1R consistently localizes near the erythrocyte plasma membrane(black arrows), but in early (B) and late (C) trophozoite-stage parasites, a subpopulation of 4.1R is found in close apposition to the parasite, asdetected by DAPI staining (blue).Parish LA, Mai DW, Jones ML, Kitson EL, Rayner JC. A member of the Plasmodium falciparum PHIST family binds to the erythrocyte cytoskeleton component band 4.1. Malar J. 2013 12(1):160.