Apical expression of RhopH1/Clag members -2, -3.1, and -9 in P. falciparum segmented schizonts. Schizont-infected erythrocytes were duallabeled with a-CL3.1B and a-RhopH2 mAb 61.3 in the 3D7 parasite line (Panel A); a-CL3.1A and a-CL9M (Panel B) and a-CL3.1A and a-CL2N (Clag2) (Panel C) in Dd2 parasite line. Overlaid images are shown in the right-hand panels. All segmented schizont-stage parasites are positive for the antisera against Clag2, -3.1, and -9. Nuclei are counterstained with either DAPI or Hoechst-33342 (Hx). Scale bar represents 5 mm. Overlaid images of the double-staining for anti-Clag3.1 and anti-PfRhopH2 or anti-Clag3.1 and anti-Clag9 sera reacting with segmented schizonts showed indistinguishable patterns of localization for these proteins. Clag3.1 and Clag2 are localized within the rhoptries of segmented schizonts. Kaneko O, Yim Lim BY, Iriko H, Ling IT, Otsuki H, Grainger M, Tsuboi T, Adams JH, Mattei D, Holder AA, Torii M. Apical expression of three RhopH1/Clag proteins as components of the Plasmodium falciparum RhopH complex. Mol Biochem Parasitol. 2005 143(1):20-8.