Stage-specific subcellular localization of PfFKBP35 and PfCnA proteins. Stages of parasite growth are indicated on the right. Both proteins (green) were probed with their primary antibody followed by FITC-conjugated secondary antibody. Nuclei (red) were stained with TO-PRO-3 iodide. All panels are super-impositions of the two stains, showing intracellular location of the proteins relative to the nucleus. Note that yellow or orange indicatesco-localization of green and red, signifying nuclear location of the protein, whereas green indicates cytoplasmic location. A significant amount of PfFKBP35 translocated to the nucleus as the rings differentiated into trophozoites and schizonts. PfCN, on the other hand, remained essentially in the cytoplasm when expressed. Kumar R, Adams B, Musiyenko A, Shulyayeva O, Barik S. The FK506-bindingprotein of the malaria parasite, Plasmodium falciparum, is a FK506-sensitive chaperone with FK506-independent calcineurin-inhibitory activity. Mol Biochem Parasitol. 2005 141(2):163-73. Copyright Elsevier