Normal numbers of midgut sporozoites are formed. Strong reduction in numbers of salivary gland sporozoites. The deficiency to enter mosquito salivary glands was accompanied by substantial accumulation of viable sporozoites in the mosquito haemocoel.Strong reduction of gliding motility of oocyst-derived/hemolymph sporozoites. Invasion of hepatoma cells in vitro of purified oocyst-derived/hemolymph sporozoites was strongly reduced. Infection of mice/rats by mosquito bite or by inoculation of purified oocyst-derived/hemolymph sporozoites resulted in blood stage infections.

Normal numbers of midgut sporozoites are formed. Strong reduction in numbers of salivary gland sporozoites. Strong reduction of gliding motility of oocyst-derived/hemolymph sporozoites. Infection of mice/rats by mosquito bite or by inoculation of purified oocyst-derived/hemolymph sporozoites resulted in blood stage infections.

Normal numbers of midgut sporozoites are formed. Strong reduction in numbers of salivary gland sporozoites. Salivary gland sporozoite numbers for the mutant at day 14 after the infected meal were dramatically reduced (90% reduction) compared to wild type sporozoite numbers. Fluorescence microscopy observation of mutant-infected salivary glands suggested that the sporozoites were mainly attached to the glands but did not localize to the interior of the gland. Infection of mice by bite of infected mosquitoes did not result in blood stage infections.Injection of 10(5) oocyst-derived or 2 x 10(4) hemolymph-derived mutant sporozoites intravenously into mice, resulted blood-stage parasitemia in all mice on the same day (day 4) as the wild type-sporozoite control-injected mice.

Invasion of hepatoma cells in vitro of purified oocyst-derived/hemolymph sporozoites was strongly reduced. Infection of mice/rats by mosquito bite or by inoculation of purified oocyst-derived/hemolymph sporozoites resulted in blood stage infections, comparable to wild type

Infection of mice/rats by mosquito bite or by inoculation of purified oocyst-derived/hemolymph sporozoites resulted in blood stage infections.

Infection of mice by bite of infected mosquitoes did not result in blood stage infections.Injection of 10(5) oocyst-derived or 2 x 10(4) hemolymph-derived mutant sporozoites intravenously into mice, resulted blood-stage parasitemia in all mice on the same day (day 4) as the wild type-sporozoite control-injected mice.