Localization of the PfI2. Expression and localization of PfI2-GFP throughout the erythrocytic cell cycle of P. falciparum. Parasites were transfected with pARL2 (construct mediating the episomal expression of full-length GFP-fused PfI2)-PfI2-GFP construct and live transfectants were analysed by fluorescence microscopy. The signal was confined within the parasite where the distribution seemed to be nucleo-cytoplasmic, as the fluorescence partially overlapped DNA staining. The distribution appeared to be diffuse in the late parasite stages with most staining in the nucleus. Frèville A, Cailliau-Maggio K, Pierrot C, Tellier G, Kalamou H, Lafitte S, Martoriati A, Pierce RJ, Bodart JF, Khalife J. Plasmodium falciparum encodes a conserved active inhibitor-2 for Protein Phosphatase type 1: perspectives for novel anti-plasmodial therapy. BMC Biol. 2013 11(1):80. .