Live cell microscopy images of the endogenously GFP-tagged candidate PF3D7_0629200 (PFF1415c DnaJ protein) show PV location. The top microscopy panel row shows a ring and a trophozoite stage, the middle row shows a schizont and the bottom row shows a late schizont. The protein is not expressed in ring stages but in trophozoites and showed a signal in the parasite periphery consistent with a PV location. In addition pronounced foci in close proximity of the nucleus weredetected, suggesting some protein remaining in the secretory pathway. It is therefore likely a protein found within the parasite and peripherally attached at the outer face of the PPM or the inner face of the PVM of trophozoitesKhosh-Naucke M, Becker J, Mesén-Ramírez P, Kiani P, Birnbaum J, Fröhlke U, Jonscher E, Schlüter H, Spielmann T. Identification of novel parasitophorous vacuole proteins in P. falciparum parasites using BioID. Int J Med Microbiol. 2017 Jul 27. [Epub ahead of print]

Properties of proteins conserved , DnaJ and 11. (A) Saponin lysis during microscopy of a cell expressing PF11_0246. The identical infected RBC is shown before (top row) and after (bottom row) lysis with saponin. (B) Released merozoites of parasites expressing PF11_0246 show peripheral fluorescence (arrowhead) in addition to a prominent focus within the parasite. Saponin treatment did not release the PV fluorescence (A). In some merozoites a peripheral staining was detected after their release from the host cell, indicating that this protein may be integral to the PPM (B).(C,D) Saponin lysis during microscopy of a cell expressing DnaJ (C) or candidate 11; Saponin lysis of living parasites showed that the staining around the parasite was unaffected by lysis of the PVM. (D). The identical infected RBC is shown before (top rows) and after (bottom rows) lysis with saponin. Fluorescence around the parasite vanished after permeabilisation of the PVM. DIC, differential interference contrast; size bars: 5 mm.Khosh-Naucke M, Becker J, Mesén-Ramírez P, Kiani P, Birnbaum J, Fröhlke U, Jonscher E, Schlüter H, Spielmann T. Identification of novel parasitophorous vacuole proteins in P. falciparum parasites using BioID. Int J Med Microbiol. 2017 Jul 27. [Epub ahead of print] PMID: 28784333