Localisation of PfFtsH1 in a P. falciparum 3D7 transfectant line carrying C-terminal 3xHA-tagged PfFtsH1. (B) Immunofluorescence localization of PfFtsH1-HA using the anti-HA mAb and antibody against the apicoplast marker ACP. No overlap of PfFtsH1-HA signal was observed with the apicoplast marker. (C) PfFtsH1 co-localizes with the mitochondrial signal in trophozoites (upper panel) and appears as punctuate signals lining the organelle defined by the mitochondrial stain Mitotracker Red in schizonts (lower panel). (D) Confocal microscopy PfFtsH1-HA expressing parasites showing co-localisation of PfFtsH1 with the mitochondrion which is stained with Mitotracker Red. clear overlap was observed between the PfFtsH1-HA signal and the mitochondria-specific dye Mitotracker Red. When the mitochondria elongated and branched in the schizont stages, punctuate signals of PfFtsH1-HA were seen along the length of the organelle (C, lower panel).Tanveer A, Allen SM, Jackson KE, Charan M, Ralph SA, Habib S. An FtsH Protease Is Recruited to the Mitochondrion of Plasmodium falciparum. PLoS One. 2013 Sep 13;8(9):e74408.