Localization of Pfstomatin to the apical organelles of merozoites and association of parasite rhoptry protein RhopH2 . Indirect immunofluorescence assays of: (i–iii) free merozoites probed with antibodies to Pfstomatin (i; green), and Mito Tracker (ii; red); the relative lack of co-localization of these markers is shown in the merged panel iii. iv–vi intracellular schizont-stage parasites expressing GFP in the apicoplast were probed with antibodies to Pfstomatin (iv; red), GFP (v; green); the relative lack of co-localization of these markers is shown in the merged panel vi. vii–ix free merozoites probed with antibodies to: Pfstomatin (vii; green), RhopH2 (viii; red); the extent of co-localization is shown in the merged panel ix. All images shown are single optical sections. The nucleus (blue) was stained with Hoechst 33342. Scale bars correspond to 2 mm.Hiller NL, Akompong T, Morrow JS, Holder AA, Haldar K. Identification of a stomatin orthologue in vacuoles induced in human erythrocytes by malaria parasites. A role for microbial raft proteins in apicomplexan vacuole biogenesis. J Biol Chem. 2003 278:48413-21.

Localization of Pfstomatin and RhopH2 to the PVM in ring-infected erythrocytes (0–8 h post-invasion). Cells were subjected to a standard indirect immunofluorescence assay where samples were permeabilized with saponin and probed with antibodies to: Pfstomatin (Ai; green), RhopH2 (Bi; green), and the PVM marker EXP-1 (Aii and Bii; red); the merged images are shown in Aiii and Biii. In C and D, ring-infected erythrocytes were treated with tetanolysin and incubated with antibodies to: Pfstomatin (Ci; red), RhopH2 (Di; red) and the PPM marker MSP-1 (Cii and Dii; green); the merged images are shown in Ciii and Diii. In E and F, rings treated with tetanolysin and permeabilized with saponin were incubated with antibodies to: Pfstomatin (Ei; red), RhopH2 (Fi; red), and MSP-1 (Eii and Fii; green); the merged images are shown in Diii and Fiii. All images shown are single optical section. The nucleus (blue) was stained with Hoechst 33342. Scale bars represent 2 mm. Hiller NL, Akompong T, Morrow JS, Holder AA, Haldar K. Identification of a stomatin orthologue in vacuoles induced in human erythrocytes by malaria parasites. A role for microbial raft proteins in apicomplexan vacuole biogenesis. J Biol Chem. 2003 278:48413-21.