Co-localization of PfASP in late stage P. falciparum schizonts with microneme protein, EBA-175 (A); rhoptry bulb protein, Clag3.1 (B); and rhoptry neck protein, PfAARP (C). P. falciparum schizonts were stained with anti-PfASP-RIV mouse sera (green) and either anti-EBA-175 (red), anti-CLAG3.1 (red) or anti PfAARP (red) rabbit sera. Parasite nuclei were stained with DAPI (blue). PfASP did not co-localize with microneme protein EBA-175 or rhoptry bulb protein CLAG3.1 but co-localized with rhoptry neck protein PfAARP in segmented P. falciparum merozoites within late-stage schizonts. Bottom: Ultra thin sections of late-stage P. falciparum schizonts containing segmented merozoites were labelled with anti-PfASP-RIV mouse sera and gold-conjugated anti-mouse IgG. Labelling was observed in the neck of rhoptries in segmented merozoites of late-stage P. falciparum schizonts. Scale bar = 250 nm.Srivastava A, Singh S, Dhawan S, Alam MM, Mohmmed A, Chitnis CE. Localization of apical Sushi Protein in Plasmodium falciparum merozoites. Mol Biochem Parasitol. 2010 74(1):66-9 Copyright Elsevier2011