RON12 is predominantly secreted late into the nascent PV. Immunofluorescence and DIC images of merozoites fixed during invasionof erythrocytes. From left to right anti-RON12 (green), anti-RON4 depicting the moving junction (red), overlay of both with nuclear stain DAPI, DIC image and overlay of all images. Cartoon schematic is shown on the right of each panel. Arrowheads depict location of moving junction. Size bars equal 1 μm.Knuepfer E, Suleyman O, Dluzewski AR, Straschil U, O'Keeffe AH, Ogun SA, Green JL, Grainger M, Tewari R, Holder AA. RON12, a novel Plasmodium-specific rhoptry neck protein important for parasite proliferation. Cell Microbiol. 2013 Aug 12. [Epub ahead of print]

RON12 is located within the rhoptry neck.. A and B. Immunofluorescence and bright-field images of schizonts (A) and merozoites (B). Images from left to right show anti-RON12 labelling (green) followed by microneme (anti-AMA1), rhoptry (neck: anti-RON4; body: anti-RAMA) and surface (anti-MSP119) specific antibodies in red, overlay of both with DAPI-stained nuclei and the bright-field image. Size bars in (A) equal 2 μm and in (B) 1 μm. C. Immuno-electron microscopy of isolated merozoites. Localization of RON12 in the rhoptry neck of three different merozoites is shown. Size bars equal 100 nm.Knuepfer E, Suleyman O, Dluzewski AR, Straschil U, O'Keeffe AH, Ogun SA, Green JL, Grainger M, Tewari R, Holder AA. RON12, a novel Plasmodium-specific rhoptry neck protein important for parasite proliferation. Cell Microbiol. 2013 Aug 12. [Epub ahead of print]

RON12 secretion into the nascent PV during invasion follows that of the rhoptry bulb located RAP1. Immunofluorescence and DIC images of merozoites fixed during invasion of erythrocytes. From left to right: anti-RON12 (green), anti-RAP1 mAb 7H8/50 (red), overlay of both with DAPI nuclear stain, DIC image and overlay of all images. Merozoite attachment in the presence of cytochalasin D (cyto D) is indicated. A cartoon schematic is shown on the right of each panel (red line represents RAP1 secreted into the host cell). Size bars equal 1 μm.Knuepfer E, Suleyman O, Dluzewski AR, Straschil U, O'Keeffe AH, Ogun SA, Green JL, Grainger M, Tewari R, Holder AA. RON12, a novel Plasmodium-specific rhoptry neck protein important for parasite proliferation. Cell Microbiol. 2013 Aug 12. [Epub ahead of print]