Subcellular localization of fluorescent proteins in transfectants of P. falciparum. Mitochondrial localization of pSSPF2/Pf Cpn10-DsRED and pSSPF2/Pf Hsp60-GFP in a double-transfectant in the ring stage (A) and in the schizont stage (B). Fluorescent proteins (DsRED, red; GFP, green) or MitoTracker Green FM (MTG, green) were monitored by fluorescence microscopy using a DeltaVision microscope system (Applied Precision). +HT, merge with the blue signal of Hoechst 33342 indicating the position of the nucleus of each parasite. +DiC, overlay on the differential phase contrast image. Scale bar: 5 mm.Sato S, Wilson RJ. Organelle-specific cochaperonins in apicomplexan parasites. Mol Biochem Parasitol. 2005 141:133-143. Copyright Elsevier 2009.