Parasites expressing the gene indicated in each panel fused to gfp were stained with Hoechst 33342 and examined 18–36 hours after infection. Panels on the left-hand side show the overlap of the Hoechst 33342 staining and the GFP fluorescence, the panels on the right show the Hoechst 33342 staining, the GFP fluorescence and a phase contrast image of the infected cell. All images are composites of multiple consecutive optical sections. PFC0435w is seen exported to the erythrocyte. It has a transmembrane spanning domain, it displays punctate spots in the erythrocyte as well as closely associated with the PV. PF13_0090 is not detected within the erythrocyte and probably not exported.van Ooij C, Tamez P, Bhattacharjee S, Hiller NL, Harrison T, Liolios K, Kooij T, Ramesar J, Balu B, Adams J, Waters AP, Janse CJ, Haldar K. The malaria secretome: from algorithms to essential function in blood stage infection. PLoS Pathog. 2008 Jun 13;4(6):e1000084, PMID