C. In both 3D7PfsD1-1 and 3D7PfsUba1 lines, GFP and ACP signals colocalise, suggesting that both are resident apicoplast proteins. Co-transfected 3D7sD1-1 with a construct encoding either the BTS of the apicoplast resident protein ACP or the mitochondrial targeting sequence of mitochondrial PfHsp60, fused to the red fluorescent protein DsRed. D. Fixed cells co-labelled with anti-GFP antibodies, ACP-DsRed PFB0385w and GFP signals co-localise, whilst HSP60-DsRed and GFP signal do not. Thus, the full-length PfsDer1-1-GFP fusion protein is transported to the apicoplast.Spork S, Hiss JA, Mandel K, Sommer M, Kooij TW, Chu T, Schneider G, Maier UG, Przyborski JM. An unusual ERAD-like complex is targeted to the apicoplast of Plasmodium falciparum. Eukaryot Cell. 2009 ;8(8):1134-45