The distance between blocking domain and TM is important for co-blocking and BPTI sensitivity. (A-E) Representative images of live P. falciparum parasites expressing the constructs shown schematically above each panel (numbers refer to the length of the amino acids sequence between TM and blocking domain). (A-C) Parasites grown in the presence (+WR) or absence of WR (control). DIC, differential interference contrast. Size bars: 5 μmMesén-Ramírez P, Reinsch F, Blancke Soares A, Bergmann B, Ullrich AK, Tenzer S, Spielmann T. Stable Translocation Intermediates Jam Global Protein Export in Plasmodium falciparum Parasites and Link the PTEX Component EXP2 with Translocation Activity. PLoS Pathog. 2016 May 11;12(5):e1005618. “

PEXEL TM proteins require translocation for export and some PEXEL proteins can also jam the translocon. (A-C) Representative images of live P. falciparum parasites expressing the constructs shown schematically above each panel (numbers refer to the length of the amino acids sequence between TM and blocking domain). DIC, differential interference contrast. Size bars: 5 μm. A schematic of the co-block (B) and failure to coblock (C) are shown to the right.Mesén-Ramírez P, Reinsch F, Blancke Soares A, Bergmann B, Ullrich AK, Tenzer S, Spielmann T. Stable Translocation Intermediates Jam Global Protein Export in Plasmodium falciparum Parasites and Link the PTEX Component EXP2 with Translocation Activity. PLoS Pathog. 2016 May 11;12(5):e1005618