Immunofluorescence assay using anti-PFI1140w and anti-PF13_0353 antisera. Purified P. falciparum trophozoites were incubated with a 1/500 dilution of antisera recognizing coding sequences of either PFI1140w or PF13_0353. Goat anti-mouse IgG conjugated with Alexa 633 was used as a secondary antibody. Nuclear DNA was labeled with Hoescht 33258. (A) DIC image of trophozoites treated with anti-PFI1140w antiserum (B) nuclear DNA stained with Hoescht 33258 and (C) negative Alexa 633 signal. (D) Merged images of (A–C). (E) DIC image of trophozoites treated with anti-PF13_0353 antiserum, (F) nuclear DNA stained with Hoescht 33258 and (G) Alexa 633 signal recognizing anti-PF13_0353 antibody. (H) Merged images of (E–G). PF13_0353 is localized in or in close proximity to the food vacuole.Ostera G, Tokumasu F, Oliveira F, Sa J, Furuya T, Teixeira C, Dvorak J. Plasmodium falciparum: food vacuole localization of nitric oxide-derived species in intraerythrocytic stages of the malaria parasite. Exp Parasitol. 2008 120:29-38.