N-terminal domain of the PfPrp16p targets the protein to the nucleus. (A) Schematic representation of the PfPrp16-GFP chimeric constructs. Predicted nuclear localization sequence, “KRRK,” is shown as a black box at 41st–44th amino acid position. (B and C) Immunofluorescence assay based localization of the chimeric GFP proteins at (B) trophozoite and (C) schizont stages respectively. Anti-GFP (red, 1:200 dilution) and anti-PfTSN (green, 1:100 dilution) antibodies were used on thin blood smears of parasite lines. PfPrp16.1-40-GFP and PfPrp16.81-last-GFP chimeric proteins do not localize with the nuclear marker PfTSN protein in either of the developmental stages, whereas chimeric protein PfPrp16.1-80-GFP was trafficked to the nucleus in trophozoite and schizont stages.Singh PK, Kanodia S, Dandin CJ, Vijayraghavan U, Malhotra P. Plasmodium falciparum Prp16 homologue and its role in splicing. Biochim Biophys Acta. 2012 1819(11-12):1186-99

Expression of the PfPrp16p during asexual blood stages of P. falciparum. Immunofluorescence assay based localization of the PfPrp16p. Anti-PfPrp16p sera (1:200 dilution) were used to localized the protein using thin blood smears of P. falciparum ring, trophozoite and schizont stages. DAPI and anti-Pf‐Tudor-SN (PfTSN) antibody were used to localize the nucleus. PfTSN co-localizes with Pf-Tudor-SN (PfTSN), a nuclear resident protein. At the trophozoite and schizont stages the protein was detected in the nucleus as well as in the cytoplasm indicating nucleo-cytoplasmic shuttling of PfPrp16p.Singh PK, Kanodia S, Dandin CJ, Vijayraghavan U, Malhotra P. Plasmodium falciparum Prp16 homologue and its role in splicing. Biochim Biophys Acta. 2012 1819(11-12):1186-99