Parasites on coverslips were fixed with paraformaldehyde and permeabilized with 0.05% saponin. The cells were probed with PfMCM2 and PfMCM6 antibodies followed by Alexafluor 555 labeled antimouse (green, PfMCM2) and Alexafluor 647 labeled anti-rabbit (red, PfMCM6) secondary antibodies. DNA was stained with DAPI (blue). Images were analyzed by DeltaVision deconvolution microscopy. The bar indicates 5mM. PfMCM2, 6, and 7 appear localized in both cytosolic and nucleosolic fractions during all intraerythrocytic stages of P. falciparum development, with increased nuclear localization in schizonts. Patterson S, Robert C, Whittle C, Chakrabarti R, Doerig C, Chakrabarti D. Pre-replication complex organization in the atypical DNA replication cycle of Plasmodium falciparum: characterization of the mini-chromosome maintenance (MCM) complex formation. Mol Biochem Parasitol. 2006 145:50-9. Copyright Elsevier 2009.