The mutant showed a slight delay in initial proliferation of blood stages in mice.

Mutants produced lower numbers of oocysts in comparison with wild type parasites (although the differences were not significant). Mutant oocysts were significantly smaller than wild type oocysts and no sporozoites were formed.

Mutant oocysts numbers in Anopheles stephensi, counted at day 10-12 post infection, were slightly lower than those produced by wild type parasites and the size of mutant oocysts at day 10-12 was significantly smaller compared to the size of wild type oocysts with a maximum size that is comparable to immature 6-8 days wt oocysts.The small oocysts that are present at day 10-12 showed a highly vacuolated cytoplasm and the absence of sporozoite formation. No sporozoites were observed in A. stephensi salivary glands at day 21-22 post infection

Mutant oocysts numbers in Anopheles stephensi, counted at day 10-12 post infection, were slightly lower than those produced by wild type parasites and the size of mutant oocysts at day 10-12 was significantly smaller compared to the size of wild type oocysts with a maximum size that is comparable to immature 6-8 days wt oocysts.The small oocysts that are present at day 10-12 showed a highly vacuolated cytoplasm and the absence of sporozoite formation. No sporozoites were observed in A. stephensi salivary glands at day 21-22 post infection

No production of oocyst- or salivary gland sporozoites

The small oocysts that are present at day 10-12 showed a highly vacuolated cytoplasm and the absence of sporozoite formation. No sporozoites were observed in A. stephensi salivary glands at day 21-22 post infection

The small oocysts that are present at day 10-12 showed a highly vacuolated cytoplasm and the absence of sporozoite formation. No sporozoites were observed in A. stephensi salivary glands at day 21-22 post infection

No production of oocyst- or salivary gland sporozoites. Infected mosquitoes were unable to infect mice by mosquito bite