Subcellular distribution of proteins predicted to be involved in invasion. All proteins were localized in schizonts (s) and free merozoites (m) using GFP-fusion proteins and grouped according to their predominant GFP localization. Shown are proteins that revealed cytosolic (PFC0945w, PFI1565w’PF11_0190 and PFL2100w, apicoplast (PFE0145w), mitochondrial and other subcellular distribution but may be involved in invasion.Hu G, Cabrera A, Kono M, Mok S, Chaal BK, Haase S, Engelberg K, Cheemadan S, Spielmann T, Preiser PR, Gilberger TW, Bozdech Z. Transcriptional profiling of growth perturbations of the human malaria parasite Plasmodium falciparum. Nat Biotechnol. 2010 28(1):91-8.

Schizont and merozoite culture was incubated overnight with 0.1µM cytochalasin D to inhibit invasion. Thin smeared on a glass slide, fixed in methanol, stained with rabbit anti-Profilin polyclonal serum 1:300 and secondary Alexa-Flor488 anti-Rabbit. Dual color fluorescence images were captured using a Carl Zeiss Axioskop 2 microscope at 100x using Oil immersion. Nucleus is marked with DAPI. Scale bar indicate 1µM. Profilin is cytoplasmic but has a trend of localization towards the apex of merozoite.Baum J, Tonkin CJ, Paul AS, Rug M, Smith BJ, Gould SB, Richard D, Pollard TD, Cowman AF. A malaria parasite formin regulates actin polymerization and localizes to the parasite-erythrocyte moving junction during invasion. Cell Host Microbe. 2008 Mar 13;3(3):188-98.