No significant difference in the number of kinesin-8X oocysts compared to wild-type controls at 7 dpi (days post-infection), but by 10 dpi we observed a significant reduction in mutant oocysts, which became even more significant at 14 dpi. By 21 dpi the number of GFP-positive kinesin-8X oocysts had decreased further to only 810% of the WT-GFP number. We also detected a significant decrease in the size of the kinesin-8X oocysts at 10, 14 and 21 dpi. At 21 dpi most of the kinesin-8X oocysts were dead, with diminished GFP expression and the presence of disintegrated nuclei. We also observed no viable sporozoites in these oocysts, and were unable to detect any salivary gland sporozoites.

Normal numbers of oocysts. Day 7 oocysts comparable to wild type (WT). Day 14 oocysts (strongly) reduced size compared to WT (approximately half the size of WT oocysts at 14 dpi, and even smaller by 21 dpi).

We did not observed viable sporozoites in the oocysts, and were unable to detect any salivary gland sporozoites.

Normal numbers of oocysts. Day 7 oocysts comparable to wild type (WT). Day 14 oocysts (strongly) reduced size compared to WT (approximately half the size of WT oocysts at 14 dpi, and even smaller by 21 dpi). No sporozoite production.

No infection of mice by bite of infected mosquitoes