Reduced (14x) multiplication/growth of asexual blood stages

Reduced gametocyte production. In 820cl1PbAT1-KO parasites, an average of 0.6% male gametocytemia compared to 1.32% was observed on day 3 following infection. Nearly no macrogametocytes could be detected in 820cl1PbAT1-KO, with an average of 0.07% compared to 1.71% in wild-type parasites, resulting in an altered sex ratio. The male gametocyte of 820cl1PbAT1-KO also appeared as empty and morphologically different from the wild-type ones through Giemsa staining.Reduced male gamete formation, defect in axoneme formation, no active exflagellation centers.