PBANKA_0310300 protein kinase 7 (PK7)
Disruptability [+]
| Species | Disruptability | Reference | Submitter | |
|---|---|---|---|---|
| P. berghei ANKA |
Possible |
RMgm-524 | Imported from RMgmDB | |
| P. berghei ANKA |
Refractory |
PlasmoGEM (Barseq) | PlasmoGEM | |
| P. falciparum 3D7 |
Possible |
22127061 | Theo Sanderson, Wellcome Trust Sanger Institute | |
| P. falciparum 3D7 |
Possible |
18083830 "a decrease in the rate of asexual growth in erythrocytes associated with a lower number of daughter merozoites generated per schizont, and second, a dramatic reduction in the ability to produce oocysts in the mosquito vector. A normal asexual growth rate and the ability to produce oocysts are restored if a functional copy of the PfPK7 gene is reintroduced into the PfPK7(-) parasites" |
Theo Sanderson, Wellcome Trust Sanger Institute | |
| P. falciparum 3D7 |
Possible |
22348509 Do not respond to melatonin-stimulation, but do upon complementation. |
Theo Sanderson, Wellcome Trust Sanger Institute | |
| P. falciparum 3D7 |
Possible |
USF piggyBac screen (Insert. mut.) | USF PiggyBac Screen | |
Mutant phenotypes [+]
| Species | Stage | Phenotype | Reference | Submitter |
|---|---|---|---|---|
| P. berghei ANKA | Asexual |
No difference |
RMgm-524 | Imported from RMgmDB |
| P. berghei ANKA | Gametocyte |
No difference |
RMgm-524 | Imported from RMgmDB |
| P. berghei ANKA | Ookinete |
Difference from wild-type |
RMgm-524
Ookinete numbers (in vitro) reduced to 9% of wild type |
Imported from RMgmDB |
| P. berghei ANKA | Oocyst |
Difference from wild-type |
RMgm-524
Oocyst numbers (day 12-14) reduced to 9% of wild type. The remaining oocyst are growth arrested from about day 9 |
Imported from RMgmDB |
| P. berghei ANKA | Sporozoite |
Difference from wild-type |
RMgm-524
no sporozoites |
Imported from RMgmDB |
| P. falciparum 3D7 | Asexual |
Attenuated |
18083830 "a decrease in the rate of asexual growth in erythrocytes associated with a lower number of daughter merozoites generated per schizont, and second, a dramatic reduction in the ability to produce oocysts in the mosquito vector. A normal asexual growth rate and the ability to produce oocysts are restored if a functional copy of the PfPK7 gene is reintroduced into the PfPK7(-) parasites" |
Theo Sanderson, Wellcome Trust Sanger Institute |
| P. falciparum 3D7 | Asexual |
Difference from wild-type |
22348509 Do not respond to melatonin-stimulation, but do upon complementation. |
Theo Sanderson, Wellcome Trust Sanger Institute |
| P. falciparum 3D7 | Oocyst |
Attenuated |
18083830 "a decrease in the rate of asexual growth in erythrocytes associated with a lower number of daughter merozoites generated per schizont, and second, a dramatic reduction in the ability to produce oocysts in the mosquito vector. A normal asexual growth rate and the ability to produce oocysts are restored if a functional copy of the PfPK7 gene is reintroduced into the PfPK7(-) parasites" |
Theo Sanderson, Wellcome Trust Sanger Institute |
Imaging data (from Malaria Metabolic Pathways)
Immunofluorescence detection of PfPK7 expression during erythrocytic development. The IgY against a peptide derived from PfPK7 (see Experimental procedures) and a FITC-conjugated secondary antibody were used to investigate the localization of PfPK7 during erythrocytic development. The slides were pre-stained with DAPI to visualize nuclear DNA. Gam, gametocyte; Troph, trophozoite; Schiz, schizont. Immunofluorescence microscopy using the anti-peptide antibody shows that the PfPK7 protein localizes throughout the cytoplasm of both asexual parasites and gametocytes, with some punctuated pattern apparent in the latter, which has not yet been investigated further.Dorin D, Semblat JP, Poullet P, Alano P, Goldring JP, Whittle C, Patterson S, Chakrabarti D, Doerig C. PfPK7, an atypical MEK-related protein kinase, reflects the absence of classical three-component MAPK pathways in the human malaria parasite Plasmodium falciparum. Mol Microbiol. 2005 55:184-96.
See original on MMPMore information
| PlasmoDB | PBANKA_0310300 |
| GeneDB | PBANKA_0310300 |
| Malaria Metabolic Pathways | Localisation images Pathways mapped to |
| Previous ID(s) | PB001186.02.0, PBANKA_031030, PbPK7 |
| Orthologs | PCHAS_0312400 , PF3D7_0213400 , PKNH_0407600 , PVP01_0411500 , PVX_002865 , PY17X_0310800 |
| Google Scholar | Search for all mentions of this gene |