Normal production of oocysts. Sporozoites fail to egress from midgut oocysts. Within the oocysts the sporozoites seemed to be arranged in circles whereas in wild type oocysts the sporozoites are arranged in the typical radial fashion. Mutant sporozoites displayed a continuous circular movement around a central axis, in both clockwise and anticlockwise directions. In wild type oocysts, sporozoite bending and flexing is seen on rare occasions and in general, no motility can be observed in wild type oocysts. Mutant oocysts were more resistant to permeabilization by detergents (1% saponin) compared to wild type oocysts.

Sporozoites fail to egress from midgut oocysts. No sporozoites were detected in the hemocoel or in the salivary glands of infected mosquitoes despite efficient infection rates and high numbers of oocyst sporozoites. Mechanically liberated sporozoites from oocysts show gliding motility. Infection of Sprague/Dawley rats by intravenous injection of large numbers of mechanically liberated sporozoites (100.000) did not result in blood stage infections.

Infection of Sprague/Dawley rats by intravenous injection of large numbers of mechanically liberated sporozoites (100.000) did not result in blood stage infections.