Oocyst development delayed

Wildtype-like oocyst production. Evidence is presented for a reduced (delayed) egress of sporozoites from the oocysts and a defect in salivary gland invasion. Strongly reduced numbers of salivary gland sporozoites.

Strongly reduced sporozoite numbers in vivo with a strong developmental delay

Evidence is presented for a reduced (delayed) egress of sporozoites from the oocysts and a defect in salivary gland invasion. Strongly reduced numbers of salivary gland sporozoites.Mice injected with wild-type sporozoites showed a prepatent period of 4 days and 7 days for salivary glands sporozoites and hemolymph sporozoites, respectively, and they did not survive due to high parasitemia beyond days 16 and 18 post sporozoite injection, respectively. However, all mice injected with knockout sporozoites did not develop any blood-stage parasitemia till day 21 post sporozoite injection.

No transmission by bite of infected mosquitoes

Mice injected with wild-type sporozoites showed a prepatent period of 4 days and 7 days for salivary glands sporozoites and hemolymph sporozoites, respectively, and they did not survive due to high parasitemia beyond days 16 and 18 post sporozoite injection, respectively. However, all mice injected with knockout sporozoites did not develop any blood-stage parasitemia till day 21 post sporozoite injection.