Disruptability [+]

Species Disruptability Reference Submitter
P. yoelii yoelii 17X
Possible
RMgm-977 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-1468 Imported from RMgmDB
P. falciparum 3D7
Possible
27324409 Theo Sanderson, Wellcome Trust Sanger Institute

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. yoelii yoelii 17X Asexual
No difference
RMgm-977 Imported from RMgmDB
P. yoelii yoelii 17X Sporozoite
No difference
RMgm-977 Imported from RMgmDB
P. yoelii yoelii 17X Liver
Difference from wild-type
RMgm-977
Normal infection of liver cells by sporozoites. Normal development of liver stages up to 24 hours. Aberrant formation of liver-stage merozoites. No viable liver-stage merozoites are formed. No blood stage infection after infection of mice with mutant sporozoites.
Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-1468 Imported from RMgmDB
P. falciparum 3D7 Asexual
No difference
27324409 Theo Sanderson, Wellcome Trust Sanger Institute
P. berghei ANKA Ookinete
No difference
RMgm-1468 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-1468 Imported from RMgmDB
P. berghei ANKA Sporozoite
No difference
RMgm-1468 Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-1468
Reduced infectivity of sporozoites as shown by infection of mice by mosquito bite or injection of purified sporozoites (prolonged prepatent period and/or reduced number of mice developing blood infections) Pb apiG3PAT (-) and wild type sporozoites did not differ in their ability to invade hepatocytes. No difference in mean parasite size was observed between Pb apiG3PAT (-) and wild type parasites at 24 hours post-infection. However, at 48 and 66 hours post-infection, Pb apiG3PAT (-) parasites were significantly smaller than wild type (with mean areas 22 % and 26 % less than the control). Wild type parasites produced the majority of detached cells and merosomes at 66 hours post-infection, with smaller numbers of these forms detected at the 72 hours. By contrast, Pb apiG3PAT parasites were never seen to produce detached cells or merosomes at either 66 or 72 hours. Pb apiG3PAT (-) parasites had noticeably smaller apicoplasts and fewer nuclei than wild type at 48 and 66 hours. Normal MSP1 expression at 48h. At 66 hours, with MSP1 staining typically detected around individual merozoites in the wild type, but remaining patchy or indicative of only limited plasma membrane invagination in Pb apiG3PAT (-) parasites.
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

Pf apiG3PAT (-) parasites have normal apicoplast morphology and development in the blood stage. A. Immunofluorescence microscopy of Pf apiG3PAT (-) and wild type parasites using antibodies against the apicoplast marker ACP demonstrates disruption of the enzyme has no effect apicoplast morphology in rings, trophozoites or schizonts. DNA stained with DAPI. Scale 3 μm. B. Transmission electron microscopy of trophozoite stage Pf apiG3PAT (-) and wild type parasites confirms disruption of the enzyme has no effect on apicoplast structure or membrane organization. Magnified images (inset) show the four apicoplast membranes are arranged in pairs (arrowheads) in both lines. Scale 100 nm.Shears MJ, MacRae JI, Mollard V, Goodman CD, Sturm A, Orchard LM, Llinás M, McConville MJ, Botté CY, McFadden GI. Characterization of the Plasmodium falciparum and P. berghei glycerol 3-phosphate acyltransferase involved in FASII fatty acid utilization in the malaria parasite apicoplast. Cell Microbiol. 2016Jun 20. PMID: 27324409.

See original on MMP

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