Disruptability [+]

Species Disruptability Reference Submitter
P. yoelii yoelii 17X
Possible
RMgm-4109 Imported from RMgmDB
P. yoelii yoelii 17X
Possible
RMgm-183 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-599 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-598 Imported from RMgmDB
P. berghei ANKA
Possible
PlasmoGEM (Barseq) Imported from PlasmoGEM

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. yoelii yoelii 17X Asexual
No difference
RMgm-4109 Imported from RMgmDB
P. yoelii yoelii 17X Asexual
No difference
RMgm-183 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-4109 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-183 Imported from RMgmDB
P. yoelii yoelii 17X Ookinete
No difference
RMgm-4109 Imported from RMgmDB
P. yoelii yoelii 17X Ookinete
No difference
RMgm-183 Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
No difference
RMgm-4109 Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
No difference
RMgm-183 Imported from RMgmDB
P. yoelii yoelii 17X Sporozoite
No difference
RMgm-4109 Imported from RMgmDB
P. yoelii yoelii 17X Sporozoite
Difference from wild-type
RMgm-183
Normal numbers of salivary gland sporozoites are produced. Sporozoites are not infectious to mice as shown by the absence of blood infections after inoculation of 50.000 sporozoites.
Imported from RMgmDB
P. yoelii yoelii 17X Liver
Difference from wild-type
RMgm-4109
Growth arrest of late liver stages
Imported from RMgmDB
P. yoelii yoelii 17X Liver
Difference from wild-type
RMgm-183
Normal numbers of salivary gland sporozoites are produced. Sporozoites are not infectious to mice as shown by the absence of blood infections after inoculation of 50.000 sporozoites. At 12 and 24 h after injection of sporozoites liver stages showed normal development that was indistinguishable from wild type, i.e. they invaded hepatocytes, formed a parasitophorous vacuole (PV), transformed into trophozoites and initiated schizogony. However, by 44 h the size of the liver stages was significantly less than that of wild type. In addition, abnormal progression of nuclear division became apparent. No expression of MSP1 was detected. No mature merozoites are formed and released, resulting in the absence of blood infection.
Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-599 Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-598 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-599 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-598 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-599 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-598 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-599 Imported from RMgmDB
P. berghei ANKA Oocyst
No difference
RMgm-598 Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-599
Normal numbers of salivary gland sporozoites are formed. Sporozoites have a reduced infectivity to mice as shown by a significant delay of blood infections (prolonged prepatent period) after intravenous inoculation of sporozoites.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-598
Normal numbers of salivary gland sporozoites are formed. Sporozoites have a reduced infectivity to mice as shown by a significant delay of blood infections (prolonged prepatent period) after intravenous inoculation of sporozoites.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-599
Normal numbers of salivary gland sporozoites are formed. Sporozoites have a reduced infectivity to mice as shown by a significant delay of blood infections (prolonged prepatent period) after intravenous inoculation of sporozoites.Sporozoites showed normal cell traversal, hepatocyte invasion rates and initial stages of intrahepatic development. The formation of merozoites within the liver schizonts was affected (see 'Additional information'). Intravenous injection of 50.000 sporozoites resulted in breakthrough blood infections in the majority (80100%) of BALB/c mice. All C57BL/6 mice developed a blood stage infection when infected with 50.000 sporozoites. The blood infections show a prolonged prepatency period of 12 days as compared to WT parasites. Assuming a P. berghei blood stage multiplication rate of 10 per 24 h this delay to patency indicates a 9099% reduction in the production and/or infectivity of the fabb/f exo-erythrocytic merozoites.
Imported from RMgmDB
P. berghei ANKA Liver
Difference from wild-type
RMgm-598
Normal numbers of salivary gland sporozoites are formed. Sporozoites have a reduced infectivity to mice as shown by a significant delay of blood infections (prolonged prepatent period) after intravenous inoculation of sporozoites.Sporozoites showed normal cell traversal, hepatocyte invasion rates and initial stages of intrahepatic development. The formation of merozoites within the liver schizonts was affected (see 'Additional information'). Intravenous injection of 50.000 sporozoites resulted in breakthrough blood infections in the majority (80100%) of BALB/c mice. All C57BL/6 mice developed a blood stage infection when infected with 50.000 sporozoites. The blood infections show a prolonged prepatency period of 12 days as compared to WT parasites. Assuming a P. berghei blood stage multiplication rate of 10 per 24 h this delay to patency indicates a 9099% reduction in the production and/or infectivity of the fabb/f exo-erythrocytic merozoites.
Imported from RMgmDB

More information