Disruptability [+]

Species Disruptability Reference Submitter
P. yoelii yoelii 17X
Possible
RMgm-4394 Imported from RMgmDB
P. berghei ANKA
Possible
RMgm-4114 Imported from RMgmDB

Mutant phenotypes [+]

Species Stage Phenotype Reference Submitter
P. yoelii yoelii 17X Asexual
No difference
RMgm-4394 Imported from RMgmDB
P. yoelii yoelii 17X Gametocyte
No difference
RMgm-4394 Imported from RMgmDB
P. yoelii yoelii 17X Ookinete
No difference
RMgm-4394 Imported from RMgmDB
P. yoelii yoelii 17X Oocyst
Difference from wild-type
RMgm-4394
Normal numbers of oocysts. Sporozoite formation inside oocysts. No salivary gland sporozoites. Oocyst-derived sporozoites are not infective to mice.
Imported from RMgmDB
P. yoelii yoelii 17X Sporozoite
Difference from wild-type
RMgm-4394
Normal numbers of oocysts. Sporozoite formation inside oocysts. No salivary gland sporozoites. Oocyst-derived sporozoites are not infective to mice.
Imported from RMgmDB
P. berghei ANKA Asexual
No difference
RMgm-4114 Imported from RMgmDB
P. berghei ANKA Gametocyte
No difference
RMgm-4114 Imported from RMgmDB
P. berghei ANKA Ookinete
No difference
RMgm-4114 Imported from RMgmDB
P. berghei ANKA Oocyst
Difference from wild-type
RMgm-4114
Normal numbers of oocysts with a size comparable to wild type oocysts. 'Normal' sporozoite formation inside oocysts. Sporozoites inside oocysts are immotile. No release of sporozoites from oocysts.
Imported from RMgmDB
P. berghei ANKA Sporozoite
Difference from wild-type
RMgm-4114
Normal numbers of oocysts with a size comparable to wild type oocysts. 'Normal' sporozoite formation inside oocysts. Sporozoites inside oocysts are immotile. No release of sporozoites from oocysts. No (very few) salivary gland sporozoites. Mechanically released sporozoites are not infectious to mice.
Imported from RMgmDB

Imaging data (from Malaria Metabolic Pathways)

PfAP2Tel localizes to telomeric foci at the nuclear periphery of blood stage parasites in vivo. B) Immunofluorescence assays were performed with anti-PfAP2Tel antibodies (red) in ring (R) and schizont (S) stage P. falciparum parasites. Nuclear DNA was stained with DAPI (blue). Confirmed nuclear distribution of PfAP2Tel. PfAP2Tel localized to the nuclear periphery as discrete foci; this pattern was especially evident in the schizont stage. C) Immunofluorescence assays with anti-PfAP2Tel antibodies (green) were combined with DNA Fluorescence in situ hybridization analysis of telomere ends (red) in ring (R) stage parasites. Nuclear DNA was stained with DAPI (blue). For B and C, scale bars indicate 5 μm (Rings) and 1 μm (Schizonts). PfAP2Tel co-localized with telomeric clusters at the nuclear periphery 80% of the time (N=50 cells). Overall, these results show that PfAP2Tel localizes to a compartment similar to telomeric clusters throughout the parasite blood stage life cycle.Sierra-Miranda M, Vembar SS, Delgadillo DM, Ávila-López PA, Vargas M, Hernandez-Rivas R. PfAP2Tel, harbouring a non-canonical DNA-binding AP2 domain, binds to Plasmodium falciparum telomeres. Cell Microbiol. 2017 [Epub ahead of print]

See original on MMP

More information